technology

Evalution™

The Evalution platform is composed of 3 major components:

  • Barcoded microparticles
    • Particles with different codes can have different biomolecules attached (antibodies, nucleic acid probes, antigens, other molecules), allowing multiple assay types and formats
    • Thousands of different codes available
  • Microfluidic assay cartridges
    • Multiple microparticle families can be loaded in 1 to 16 microfluidic channels of an assay cartridge, allowing the creation of a multiplex assay environment
    • Microfluidics allow improved binding kinetics and therefore fast assays
    • Random access: 1 to 16 multiplex assays can be run simultanously
  • Evalution instrument
    • Once loaded into the Evalution instrument, all assay steps (sample application, incubations, washes, detection) are run in the instrument, allowing full workflow integration
    • All assays are run in a fully controlled environment and are therefore very robust

 

Evalution_parts

 

 

A broad range of technological features combined in one platform

Evalution™ combines in one platform multiple technological features.

Evalution_techfeat

  • Multiplex
    • A multiplex level of 150 analytes per channel can be reached
  • Multipurpose
    • Different biomolecules can be coupled to the microparticles: antibodies, antigens, nucleic acid probes, carbohydrates…
    • Only one fluorophore needed to detect all targets
  • Visualization
    • Immediate and live visual control at microparticle level
    • Optimal assay development tool
  • High performing
    • A fully controlled assay environment result in state-of-the-art assay specifications
    • No user-induced variation
  • Flexible
    • The 16 channels in the assay cartridge are individually controlled
    • One channel can be run on day 1, the next 5 on day 2…
    • No sample batching needed
  • Fast
    • The microfluidic environment induces a reaction-limited kinetic regime, excluding all diffusion effects
    • Short incubation times and no or limited need for wash steps result in fast assays and short turnaround times
  • Kinetics
    • Reactions can be assessed at end-point
    • Binding reactions can also be followed in real time, allowing even shorter time-to-result
  • Temperature control
    • Temperatures are independently controlled (25-95C) in three different cartridge zones: the input wells, the fluid transition zone and the detection zone

Assays with unique features

Thanks to its unique technological features, Evalution allows the development of fast and robust assays

Evalution_apps

 

  • Multiple applications
    • Multiplex assay formats: Get “syndromic” information of a patient
    • Multiple sample types: all liquified matrices (blood, CSF, stool, tissue…)
  • Fast
    • Short sample turnaround time, i.e. fast access to results
  • Robust solutions
    • High-end analytical specifications (sensitivity, specificity, reproducibility, dynamic range…)
    • Kinetic next to end-point measurements
  • Easy workflows
    • Once an assay cartridge is loaded, all assay steps occur on-board of the instrument without further manual plate handling

 

Watch the Evalution™ workflow

Click here to watch the video of a typical workflow to execute a multiplexed sandwich immunoassay from a set of encoded microparticles. The reporting molecules are flushed together with the sample for a single-injection, wash-free protocol with real-time visualization of binding reactions. More traditional protocols with sequential steps of incubation, wash, and end-point readout are also possible.

 

Found some features that could benefit your application?

Contact us for more information on the technology and its applications:

MyCartis
VIB bio-incubator
Technologiepark 4
9052 Zwijnaarde
Belgium
T +32 9 241 11 40
info@mycartis.net